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KMID : 1098420070150020100
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Korean Journal of Medicinal Crop Science
2007 Volume.15 No. 2 p.100 ~ p.104
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Factors Affecting Agrobacterium tumefaciens-mediated Transformation of Panax ginseng C.A. Meyer
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Kim Ok-Tae
Jung Su-Jin
Bang Kyung-Hwan
Kim Young-Chang
Shin Yoo-Soo
Sung Jung-Sook
Park Chun-Geon
Seong Nak-Sull
Cha Seon-Woo
Park Hee-Woon
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Abstract
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A protocol for the production of transgenic Panax ginseng C.A. Meyer was established via Agrobacterium tumefaciens-mediated genetic transformation of direct somatic embryos. A number of conditions related to the co-cultivation were tested with respect to maximizing transformation efficiency. The results showed that pH of the co-cultivation medium (5.7), the bacterial growth phase (optical density; = 0.8), co-cultivation period (3 days), and acetosyringone concentration had positive effects on transformation. Selected plantlets were cultured on the medium at an elevated hygromycin level(30 mg/l). Integration of the transgenes into the P. ginseng nuclear genome was confirmed by PCR analysis using hpt primers and by Southern hybridization using hpt-specific probe. The transgenic plantlets were obtained after 3-month cultivation and did not show any detectable variation in morphology or growth characteristics compared to wild-type plants.
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KEYWORD
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Agrobacterium tumefaciens, Panax ginseng, transformation, hygromycin
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